CAG-DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB

CAG-TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

CRISPRoff-v2.1

CRISPRon

lentiCRISPRi(v2)-Blast

lentiCRISPRi(v1)-Blast

pAAVS1-NC-CRISPRi (Gen3)

pAAVS1-NDi-CRISPRi (Gen2)

pAAVS1-NDi-CRISPRi (Gen1)

pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

dCas9-VPR_P2A_mCherry

pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

pCRISPRa_all-in-one

pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE